The two teams are set for their first ever top-flight meeting.
The youth team beat Brescia 5-1 and extend their winning run.
Mihajlovic has named a 24-man squad for the round 14 clash. Kick-off 20.45.
The club president held a lunch with sponsors on Friday at Milanello.
Milan are aiming to preserve their steely home defence record versus Samp.
Mihajlovic previewed the rossoneri’s league match with Sampdoria.